Fig. 5

HDAC1 is associated with augmented aging in APN-deficient mice. a: Immunoblots and their quantitative column graphs, showing expression of HDAC1, 2, 3, H3K9me3, 2, and 1, in the cerebral cortex of age-matched APN KO vs. WT mice (n = 4–5). b: Representative immunoblots and bar graphs show p16, p21, and HDAC1 expression in AdipoRon-, Rot- and Anti A-treated BV2 cells (n = 3). c-d: Bar graphs showing the level of ATP and ROS in AdipoRon-, Rot- and Anti A-treated BV2 cells (n = 3). e: ATP level in the Anti A- and Cpd-50-treated primary microglial cells from WT and APN KO mice (n = 3). f: Representative immune blots and bar graphs showing relative expression of p16, p21, and HDAC1. Immunoblot densities were optimized by standard (GAPDH/β-actin) (n = 3). Data expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001