Fig. 3
From: The effect of metformin on influenza vaccine responses in nondiabetic older adults: a pilot trial
Flow cytometry analysis of circulating PBMC responses to flu vaccination. Peripheral blood mononuclear cells (PBMCs) were analyzed for A) circulating T follicular helper cells (cTfh), B) activated ICOS + PD1 + cTfh, and C) Antibody Secreting Cells (ASCs). Data are presented as fold change at given week post vaccination compared to pre-vaccination levels. A trending main metformin treatment effect was observed in cTfh. Statistical significance was calculated by two-way repeated measures ANOVA with Å Ãdák’s posthoc corrections and significance set at p < 0.05