Fig. 1

Age-depended glycosphingolipid accumulation and lysosomal dysfunctions in male GlaKO mouse DRG. A Gb3 was identified using indirect immune fluorescence microscopy using anti-Gb3 (CD77) in DRG from adult (28 weeks) and old (57 weeks) mice. Scale bar 50 µm. B Quantification of Gb3 accumulation. 28 weeks wt mice n = 6, GlaKO mice n = 7, 57 weeks wt mice n = 6, GlaKO mice n = 8. Statistical significance was assessed using Kruskal–Wallis test followed by pairwise comparisons using Wilcoxon rank sum exact test and Bonferroni correction. C 3D reconstruction of MP detail images from neuronal cell bodies and (D) axon bundles, showing in detail the localization of Gb3 depositions. Scale bar 10 µm. E Representative images of LAMP1 staining and quantification of increased lysosomal distribution and cell body area of isolated DRG neurons. Scale bar 5 µm. n = 22 cells per group. F Time-lapse analysis of lysosome trafficking in DRG neurons and track length measurements. n = 20 cells per group. Mann–Whitney U test. Values are given as mean ± SEM.* p < 0.05, ** p < 0.01, *** p < 0.001, if not otherwise stated