Fig. 3

Sex-dependant increase in pro-inflammatory and chemotactic factor in Taok3 deficient mice. a Serum cytokine and chemokine measurements in young and aged mice reported by fold change (KO/WT within the same age group) and associated p-value. b Bar chart illustrating CXCL9 (left) and TNFα (right) levels, stratified by the sex, age, and genotypes of the animals. c (from top left to bottom right) Quantifying monocytic migration potential in a model of acute peritonitis in vivo. The gating strategy was used to define the major B cell (CD19 + , CD11b-) and CD11b + populations found in the peritoneal cavity in steady state and during Thioglycollate (Thio.) induced peritonitis. Bar chart representing the distributions of B cells and CD11b + cells within the peritoneal cavity. These experiments were conducted in female mice. d Gating strategy to characterize resident large peritoneal macrophages (LPMs) from recruited small peritoneal macrophages (SPMs) and recruited monocytes (mono) within the CD11 + population. Associated bar chart representing the frequency of each subpopulation. a-b Data representative of n = 5 animals/genotype/sex/age-group. c-d Data representative of independent experiments conducted on a total of n = 6 mice/condition/genotype. Chart error bars represent mean ± SEM. Statistical analysis: a-d Multiple unpaired, bilateral T-tests. ns, non-significant. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001