Fig. 1

Immunophenotyping of DCs. DCs phenotype and activation, maturation and exhaustion markers and intracellular production of TNFα on DCs. Bar and scatter dots graphs represent the expression of each biomarker in total DCs: CD40, CD80, TIM-3, PDL-1 (A.1-A.4); TNFα intracellular production (A.5); HLA-MFI (A.6). The bar graphs represent marker expression: CD40 on plasmacytoid dendritic cells (pDCs) (B); CD80 on myeloid dendritic cells (mDCs) (C); CD80 on pDCs (D) and intracellular production of TNFα on pDCs (E). The medians with the interquartile ranges are shown. Ex-vivo: Ex-vivo condition; UT: Untreated condition; α1Thy: α1Thy treated condition. Each dot represents an individual. Orange dots represent < 40 years (n = 18) and > 65 years (n = 16) are highlighted with blue dots. Wilcoxon test was used comparing condition in the same group and U-Mann Whitney test was used comparing condition between different group (ns: not statistically significative, θ: p > 0.05 and < 0.1 *p < 0.05; **p < 0.01; ***p < 0.001)