Fig. 2

Immunophenotyping and cytokine production on total CD4 + and CD8 T cells. Intracellular production of TNFα, IFNγ, and IL-2 in T cells was evaluated from co-culture of CD4 + or CD8 + TLs in response to SARS-CoV2 peptides with autologous DCs previously treated with α1Thy. Bar and Scatter plots graphs represent percentage of cytokine production: in total CD4 TLs TNFα, IFNγ, and IL-2 (A-C); in total CD8 TLs: TNFα, IFNγ, and IL-2 (D-F). The medians with the interquartile ranges are shown. Ex-vivo: Ex-vivo condition; UT: Untreated condition; α1Thy: α1Thy treated condition; SARS-CoV2: SARS-CoV2 peptides stimulation condition; α1Thy + SARS-CoV2: α1Thy treated and SARS-CoV2 peptides stimulation condition. Each dot represents an individual. Orange dots represent < 40 years (n = 18) and > 65 years (n = 16) are highlighted with blue dots. Wilcoxon test was used comparing condition in the same group and U-Mann Whitney test was used comparing condition between different group (ns: not statistically significative, θ: p > 0.05 and < 0.1 *p < 0.05; **p < 0.01; ***p < 0.001)