Fig. 3

Immunophenotyping and cytokine production on CD4 + and CD8 memory T cells. Intracellular production of TNFα, IFNγ, and IL-2 in T cells was evaluated from co-culture of CD4 + or CD8 + TLs in response to SARS-CoV2 peptides with autologous DCs previously treated with α1Thy. Bar and Scatter plots graphs represent percentage of cytokine production: in memory populations of CD4 + TLs in < 40 years’ group: TNFα, IFNγ, and IL-2 (A.1-A.3); in memory populations CD8 + TLs in < 40 years’ group: TNFα, IFNγ, and IL-2 (B.1-B.3); in memory populations of CD4 + TLs in > 65 years’ group: TNFα, IFNγ, and IL-2 (C.1-C.3); in memory populations CD8 + TLs in > 65 years’ group: TNFα, IFNγ, and IL-2 (D.1-D.3). The medians with the interquartile ranges are shown. Ex-vivo: Ex-vivo condition; UT: Untreated condition; α1Thy: α1Thy treated condition; SARS-CoV2: SARS-CoV2 peptides stimulation condition; α1Thy + SARS-CoV2: α1Thy treated and SARS-CoV2 peptides stimulation condition. Each dot represents an individual. Memory populations represented in green central memory T-cells (CM), in violet effector memory T-cells (EM) and in red terminally differentiated effector memory T cells (TEMRA). Wilcoxon test was used comparing condition in the same group and U-Mann Whitney test was used comparing condition between different group (ns: not statistically significative, θ: p > 0.05 and < 0.1 *p < 0.05; **p < 0.01; ***p < 0.001)