Fig. 2

Phagocytized photoreceptor outer segments (POS) in aged RPE and subretinal microglia. A, B TEM and rhodopsin staining (rhodopsin—green, f-actin—red) revealed accumulation of undigested POS in aged RPE. C, D Phagocytosed POS are also observed inside subretinal microglia (green and orange arrow) by TEM and by rhodopsin staining (red arrows; rhodopsin—green, iba1—white). Colocalization of rhodopsin in orthogonal x–y cross sections inside the RPE and microglia(orange stars). Rhodopsin on top of the RPE or outside microglia (white stars). Images were taken from mice aged 26 – 35 months