Fig. 4

The PLX5622 diet effectively depleted P2RY12 microglia. A Study design summary showing 18-month mice were feed control or microglia depletion diet (PLX5622) for 6 weeks. B Representative high magnification images of IPL and OPL of and 18 months old control and PLX-treated mouse. C Quantification of P2RY12 + cells in IPL and OPL of control mice and mice on the depletion diet. D Representative RPE flat mounts of 18 months old mice on the control or microglia depletion diet. P2RY12 positive cells counted with ImageJ are labeled in purple. E Quantification of subretinal microglia per eye. n = 10 mice per group, unpaired t-test. **P ≤ 0.01; ****P ≤ 0.0001; ns, not significant. Scale = 50 µm