Fig. 6

Microglia-depleted mice show morphological abnormalities in the RPE. Comparison of RPE A cell perimeter, B cell area, C cell density, and D percentage of multinucleated (≥ 3 nuclei per cell)of control versus microglia-depleted mice. E Representative images of RPE flat mounts acquired in the mid-periphery region and stained with F-actin (red) and DAPI (blue) show a regular hexagonal morphology with either single or binucleated cell in control compared to the enlarged and swollen RPE morphology with multinucleated cells (white stars) in microglia-depleted mice. Scale bar is 20 μm. n = 3 mice, unpaired t-test. *P ≤ 0.05; **P ≤ 0.01