Fig. 2

Proteomic profiling of STIF-triggered immune responses of red blood cells to pathogen infection. (A) The top ten KEGG pathways enriched in red blood cells by STIF were obtained by GSEA. The pathway marked by the purple pentagram (★) is involved in the KEGG human disease database. The colormap corresponds to -log₁₀(P value) of GSEA. The bubble size indicates the number of proteins annotated to a KEGG pathway. The horizontal axis is the value of NES (normalized enrichment score) in GSEA. The vertical axis is set as the names of KEGG pathways. (B) Pathways involved in the KEGG human disease database were obtained by GSEA, and disease categories are distinguished by color. Infectious diseases are marked in blue, including viral, bacterial and parasitic diseases. Other diseases are marked in green, including immune disease, endocrine and metabolic disease, substance dependence and cancer. Pathways were filtered by P value < 0.05 and |NES|≥1. The colormap corresponds to -log₁₀(P value) of GSEA. The horizontal axis is the value of NES in GSEA. The vertical axis is set as the names of KEGG human disease pathways. (C) Venn diagram depicting eleven intersecting proteins in at least two pathways, nine of which are involved in the complement system