Fig. 3
From: Obesity accelerates age defects in B cells, and weight loss improves B cell function
Obesity and aging induce similar changes in the expression of transcription factors for class switch and these changes can be reversed, at least in part, by weight loss. B cells were isolated from PBMCs using CD19 microbeads and positive selection. B cells (106 cells/ml) were cultured with CpG for 1 day. The mRNA was extracted and qPCR performed to evaluate expression of E47 (A) and T-bet (B) mRNA, respectively. Results show qPCR values which are measures of RNA expression of target genes, relative to the housekeeping gene GAPDH, calculated as 2−ΔCts. Mean comparisons between groups were performed by two-way ANOVA: *p < 0.05, **p < 0.01, ****p < 0.0001