Fig. 4
From: Obesity accelerates age defects in B cells, and weight loss improves B cell function
Obesity, similar to aging, increases blood frequencies of inflammatory DN B cells, associated with reduced responses to the influenza vaccine and with increased autoimmunity, which are then decreased by weight loss. Unstimulated PBMCs were membrane stained with Live/Dead detection kit and anti-CD45/CD19/CD27/IgD antibodies. Top. Gating strategies to evaluate the major B cell subsets. Bottom. Frequencies of naïve, IgM memory, switched memory and DN B cells from all donors (means ± SE), gated on live CD45 + CD19 + , are shown. Mean comparisons between groups were performed by two-way ANOVA: *p < 0.05, **p < 0.01, ****p < 0.0001