Fig. 6
From: Obesity accelerates age defects in B cells, and weight loss improves B cell function
Obesity, similar to aging, increases in vitro secretion of the pro-inflammatory cytokine IL-6 and decreases that of the anti-inflammatory IL-10, and weight loss ameliorates but does not completely reverse this secretory phenotype. B cells were isolated from PBMCs using CD19 microbeads and positive selection. B cells (106 cells/ml) were cultured with CpG for 2 days. IL-6 (A) and IL-10 (B) were measured in culture supernatants by ELISA. Mean comparisons between groups were performed by two-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001