Fig. 3
The effects of high plasma Aβ1-42 on proportions changes of mononuclear macrophage. a The alterations of monocytes proportions in Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice spleen MNCs. Monocytes were stained with CD11b+CD115+. b The alterations of macrophages proportions in Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice abdominal cavity MNCs. Macrophages were stained with CD11b+F4/80+. c The alterations of pro-inflammatory macrophages proportions in macrophages of Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice abdominal cavity. The pro-inflammatory macrophages were stained with CD40+iNOS+. d The alterations of anti-inflammatory macrophages proportions in macrophages of Wt, Tg, paWt(Wt-Wt), and paWt(Wt-Tg) mice abdominal cavity. The anti-inflammatory macrophages were stained with CD206+Arg-1.+. e The statistical diagram of monocytes proportions in Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice spleen MNCs. In Tg mice spleen mononuclear cells (MNCs) were significantly higher than Wt mice (at the age of 7 months p = 0.000, at the age of 11 months p = 0.000). After parabiosis for 4 and 8 months, the proportions of monocytes in PaWt(Wt-Tg) mice spleen MNCs were significantly higher than PaWt(Wt-Wt) mice (after parabiosis for 4 months p = 0.000, after parabiosis for 8 months p = 0.005). f The statistical diagram of macrophages proportions in Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice abdominal cavity MNCs. At the age of 7 and 11 months, the proportions of macrophages in Tg mice abdominal cavity MNCs had no significantly difference compared with Wt mice (at the age of 7 months p = 0.24, at the age of 11 months p = 0.485). The proportions of macrophages in abdominal cavity MNCs had no significantly difference in PaWt(Wt-Tg) mice compared with PaWt(Wt-Wt) mice after parabiosis for 4 and 8 months (after parabiosis for 4 months p = 0.831, after parabiosis for 8 months p = 0.027). g The statistical diagram of pro-inflammatory macrophages proportions in macrophages of Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice abdominal cavity. The proportions of pro-inflammatory macrophages in macrophages of Tg mice abdominal cavity MNCs were decreased significantly (p = 0.002) at the age of 7 months, and the proportions of pro-inflammatory macrophages in Tg mice abdominal cavity MNCs had no significantly difference compared with Wt mice at the age of 11 months (p = 0.093). The proportions of pro-inflammatory macrophages in macrophages of PaWt(Wt-Tg) mice abdominal cavity MNCs were decreased significantly after parabiosis for 4 months (p = 0.001), and the proportions of pro-macrophages had an increased tendency in PaWt(Wt-Tg) mice compared with PaWt(Wt-Wt) mice after parabiosis for 8 months (p = 0.329). h The statistical diagram of anti-inflammatory macrophages proportions in macrophages of Wt, Tg, paWt(Wt-Wt), and paWt(Wt-Tg) mice abdominal cavity. The proportions of anti-inflammatory macrophages in Tg mice abdominal cavity MNCs had an increased tendency compared with Wt mice at the age of 7 months (p = 0.699) and at the age of 11 months, the proportions of anti-inflammatory macrophage in Tg mice abdominal cavity MNCs had no significantly difference compared with Wt mice (p = 0.818). The proportions of anti-inflammatory macrophages in PaWt(Wt-Tg) mice abdominal cavity MNCs had an increased tendency compared with PaWt(Wt-Wt) mice after parabiosis for 4 months (p = 0.385), and after parabiosis for 8 months, the proportions of anti-inflammatory macrophages in PaWt(Wt-Tg) mice abdominal cavity MNCs had no significant alterations compared with PaWt(Wt-Wt) mice (p = 0.153). (Wt, Tg, and PaWt(Wt-Tg) mice n = 6; PaWt(Wt-Wt)mice n = 12, data from the two Wt mice of Pa(Wt-Wt) mice, Mean ± SD, one-way analysis of variance. ***P < 0.001, **P < 0.01, *P < 0.05, ns denotes no statistical significance)