Fig. 5
The effects of high plasma Aβ1-42 on macrophages secreting function. a The plasma levels of cytokines in Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice at the age of 7 months. At the age of 7 months, the plasma levels of pro-inflammatory cytokines IL-6 in Tg mice were decreased significantly (p = 0.04), however, the levels of pro-inflammatory cytokines IL-12p70 (p = 0.31), IL-1β (p = 0.537) and anti-inflammatory factors such as IL-10 (p = 0.244) and TGF-β (p = 0.485) in Tg mice had no significant difference compared with Wt mice. Compared with PaWt(Wt-Wt) mice, the plasma levels of IL-6 in PaWt(Wt-Tg) mice were significantly decreased after parabiosis for 4 months (p = 0.037), while the levels of pro-inflammatory cytokines IL-12p70 (p = 0.95), IL-1β (p = 0.25) and anti-inflammatory factors such as IL-10 (p = 0.733) and TGF-β (p = 0.931) had no significant difference between two groups both after parabiosis for 4 months. b The plasma levels of cytokines in Wt, Tg, PaWt(Wt-Wt), and PaWt(Wt-Tg) mice at the age of 11 months. At the age of 11 months, the plasma levels of pro-inflammatory cytokines IL-6 (p = 0.002) and TNF-α (p = 0.041) were increased significantly compared with Wt mice, however, the levels of pro-inflammatory cytokines IL-12p70 (p = 0.310), IL-1β (p = 0.132) and anti-inflammatory factors such as IL-10 (p = 0.065) and TGF-β (p = 0.093) in Tg mice had no significant difference compared with Wt mice. Compared with PaWt(Wt-Wt) mice, after parabiosis for 8 months, the plasma levels of both IL-6 (p = 0.002) and TNF-α (p = 0.005) were significantly increased in PaWt(Wt-Tg) mice, while the levels of pro-inflammatory cytokines IL-12p70 (p = 0.639), IL-1β (p = 0.701) and anti-inflammatory factors such as IL-10 (p = 0.765) and TGF-β (p = 0.525) had no significant difference between PaWt(Wt-Tg) mice and PaWt(Wt-Wt) mice. c The plasma levels of cytokines in Wt mice after tail intravenous injection with Aβ1-42 peptide and controls injected with PBS. The plasma levels of IL-6 in Wt mice were increased significantly (p = 0.000), and the levels of IL-12p70 (p = 0.385), TNF-α (p = 0.766), IL-10(p = 0.827), IL-1β (p = 0.542), and TGF-β (p = 0.24) exhibited no significant differences after tail intravenous injection with Aβ1-42. (n = 6 for each group, Mean ± SD, one-way analysis of variance.**P < 0.01, *P < 0.05, ns denotes no statistical significance)