Fig. 4
From: Towards a better preclinical cancer model – human immune aging in humanized mice
In vitro analysis of T cell function (A) Intracellular staining of splenocytes for interferon-gamma and granzyme B, 4 h after culture with or without PMA/ionomycin stimulation. CD4 and CD8 cells were distinguished by FACS. Bars show mean ± SD and p values are indicated; n = 5. B and C Analysis of cytokines in the supernatant 72 h after culturing splenocytes in the absence (B) or presence (C) of anti-CD3/28 stimulation, using LEGENDplex™ T helper cytokine panel (left) or LEGENDplex™ CD8/NK panel (right) to probe for T helper or CD8/NK related cytokines, respectively. Bars show mean ± SD and p values are indicated; n = 5. Mann–Whitney test was used for all significance tests