Fig. 5
From: Towards a better preclinical cancer model – human immune aging in humanized mice
Tumor study in aging and young humanized mice. A Schematic diagram showing reconstitution of NIKO with human immune cells and subsequent engraftment of liver cancer cell line PLC/PRF/5 in young (Week 18) and aging (Week 60) humanized mice. B Pictures of tumors harvested 4 weeks post engraftment. Scale bar, 1 cm. Change in tumor volume over time. Bars show mean ± SD and p values are indicated; n = 5. C Analysis of TILs for percentage of hCD45 and CD4/CD8 ratio. Bars show mean ± SD and p values are indicated; n = 5. D Analysis of TILs for percentage of cells expressing exhaustion markers. Bars show mean ± SD and p values are indicated; n = 5. E KLRG1 expression on CD3+ T cells in blood and spleen of non-tumor engrafted humanized mice. Bars show mean ± SD and p values are indicated; n = 5. F LEGENDplex™ analysis of serum from tumor engrafted mice using Inflammation panel 1, 4 weeks post engraftment. Bars show mean ± SD and p values are indicated; n = 5. Mann–Whitney test was used for all significance tests