Fig. 2

Autologous serum modulation of TREM2 synthesis in short- and long-term differentiated Mo-MФs and serum effect. a TREM2 mRNA and b sTREM2 levels in Mo-MФ cultures from AD patients (n = 21) and CO (n = 16) in AS. Effect of AS vs. FCS on c TREM2 mRNA and d sTREM2 levels in short-term and long-term Mo-MФs from AD patients (n = 21) and CO (n = 16). Dots represent individual participant values. a, b Closed bars and symbols represent M0 (light grey for short-term; dark grey for long-term), M1 (light blue for short-term; dark blue for long-term) and M2 (light orange for short-term; dark orange for long-term) macrophages respectively. c, d Closed violins and symbols represent AS (green) and FCS (dark grey). mRNA (normalized to GAPDH) expression was measured with RT-qPCR. The Friedman ANOVA test or Wilcoxon signed rank test (paired) was used to compare within-group differences. Significant values of ANOVA tests were then subjected to Dunn's multiple comparison test with Bonferroni correction. Statistical significance was determined at the p ≤ 0.05 level unless a Bonferroni adjustment was required for multiple comparisons) p* < 0.0167 (p/n, assuming n = 3 comparison)