Fig. 2

Transcriptional analysis of three monocyte subsets in young (24–36 years, n = 9) and aged (67–83 years, n = 9) individuals. A. Distributions of overall gene expression levels of three monocyte subsets in young and elderly individuals. Red denotes classical subset, green symbolizes intermediate subset and blue represents nonclassical subset. B. PCA plot of overall gene expression levels of three monocyte subsets in young and elderly individuals. Each point denotes a sample from one subset, indicated by color: classical subset (red), intermediate subset (green) and nonclassical subset (blue). Circle represents young individuals and triangle symbolizes aged persons. C. Heatmap of hierarchical clustering analysis of the top 100 discriminant genes independent of age for each monocyte subset: classical subset (red), intermediate subset (blue) and nonclassical subset (green). Red represents elevated gene expression and blue symbolizes decreased gene expression