Fig. 1

Workflow of the experimental design. Adipose-derived stem cells (ADSC) from human origin were maintained in culture media with 10% FBS (control) or treated with FBS supplemented with a pool of plasma (0.5%) from eutrophic volunteers (PE) or a pool of plasma (0.5%) from patients with obesity (PO) for 18 days. Experiments were done at different time points (solid lines 2, 10, and 18 days). CPD and NMA analysis were done every 6 (dashed line, triangle for CPD) or 3 days (dashed line, diamond for NMA). Abbreviations: SA-β-gal: Senescence-associated beta-galactosidase; CDKN1A: Cyclin-dependent kinase inhibitor 1 A; CDKN2A: Cyclin-dependent kinase inhibitor 2 A; CPD: cumulative population doubling; DMEM: Dulbecco’s modified Eagle’s medium; mtDNA: Mitochondrial DNA; NMA: nuclear morphometric analysis; O2^•−: Superoxide anion; p-p38MAPK: phosphorylated p38 mitogen-activated protein kinase; p-p65: phosphorylated nuclear factor kappa B NF-κB p65 subunit; PE: pool of plasma from eutrophic individuals supplemented in culture medium; PO: pool of plasma from individuals with obesity supplemented in culture medium; TRF1: Telomeric repeat factor 1