Fig. 2
Chronic exposure to plasma from patients with obesity causes cell cycle arrest in G2/M in ADSC. (A) Kinetics of CPD after 18 days of plasma exposure (n = 3). (B) Population doubling time (PDT) showing an increase in PE and PO groups, compared to control. (C) (Left) Representative backgated plots and histograms from flow cytometry analysis of cell cycle and (right) quantification of cell cycle phases. The plot shows an accumulation of ADSC cells in the G2/M phase in PO treatment after 10 days. D) CDKN1A (p21WAF1/Cip1) mRNA expression (relative to RPLP0 housekeeping gene) was quantified after 10 or 18 days of treatment. E) No difference in the (left) expression or (right) positive cells of active caspase-3 in ADSC treated with plasma or control group. Data presented as mean and standard deviation (SD). Differences were considered when p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), or p < 0.0001 (****), evaluated by one-way ANOVA followed by Tukey post-test or AUC, with a confidence interval of 95%. Abbreviations: AUC: area under the curve; CDKN1A: Cyclin-dependent kinase inhibitor 1 A; PDT: population doubling time; PE: pool of plasma from eutrophic individuals supplemented in culture medium; PO: pool of plasma from individuals with obesity supplemented in culture medium