Fig. 3

Nuclear enlargement in ADSC through obesogenic environment exposure. (A) Representative images (scale bar 50 μm) of ADSC cultured in control media or supplemented with PE or PO. Nuclei were stained with DAPI and images were acquired using an Olympus IX71 microscope on specified days. (B) (Right) Distribution of nuclei area versus NII. (C) (Left) Quantification (%) of normal (N) nuclei or (right) large and regular (LR) nuclei. (D) Relative measurement of nuclear area compared to control among treatments expressed as a fold-change. (E) Integrated analysis demonstrating CNE augment of ADSC nuclei in cells treated with PO compared to PE and control. Data presented as mean and standard deviation (SD). Differences were considered when p < 0.05 (*), p < 0.01 (**), or p < 0.001 (***), evaluated by one-way ANOVA test followed by Tukey post-test or area under the curve, with a confidence interval of 95%. Abbreviations: CNE: cumulative nuclear enlargement; NII: nuclear irregularity index; PE: pool of plasma from eutrophic individuals supplemented in culture medium; PO: pool of plasma from individuals with obesity supplemented in culture medium