Fig. 7
Plasma stimuli promote mitochondrial morphology and network remodeling. Cell-live imaging analysis in ADSC exposed to the plasma altered mitochondria morphology after 10 days of treatment. (A) Representative images analyzed with MitoTracker Red™ Red CMXRos staining (scale bar: 10 μm) using Mitochondria Analyzer plugin from ImageJ FIJI. Analysis was performed in 10–20 cells (treatment in triplicate) acquired from different fields. The following measurements are presented (B) Area (μm2); (C) Perimeter (μm); (D) Form Factor (a.u.); (E) Aspect Ratio (a.u.); (F) Branches/mitochondria; (G) Mean Branch Length (μm); and (H) Branch Junctions (μm). (I) Gene expression (relative to HPRT1 housekeeping gene) of mitochondrial fusion (MFN1 and MFN2 and OPA1) and fission (FIS1) components were up-regulated both in PE and PO compared to control. Data presented as mean and standard deviation (SD). Differences were considered when p < 0.05 (*) or p < 0.0001 (****), evaluated by one-way ANOVA test followed by Tukey post-test, with a confidence interval of 95%. Abbreviations: a.u.: arbitrary units; FIS1: Mitochondrial Fission Protein 1; MFN1: Mitofusin 1; MFN2: Mitofusin 2; OPA1: Optic Atrophy 1; PE: pool plasma of eutrophic individuals supplemented in complete culture medium; PO: pool plasma of individuals with obesity supplemented in the complete culture medium;