Fig. 8

The obesogenic environment induces the accumulation of lipid droplets. (Left: scale bar 200 μm) Image analysis showed that there was an increase in the accumulation of lipid droplets of ADSC exposed to the obesogenic environment for 10 days compared to the control group. We included a group composed of ADSC induced to adipogenic differentiation as a positive control (ADIPO). (Right) Fluorescence was quantified as arbitrary units/cell (n = 10–15 cells/treatment in triplicate). Data presented as mean and standard deviation (SD). Differences were considered when p < 0.05 (*) or p < 0.0001 (****), evaluated by one-way ANOVA test followed by Tukey post-test, with a confidence interval of 95%. Abbreviations: PE: pool plasma of eutrophic individuals supplemented in the complete culture medium; PO: pool plasma of individuals with obesity supplemented in the complete culture medium; ADIPO: ADSC induced to adipogenic differentiation for 10 days