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Fig. 3 | Immunity & Ageing

Fig. 3

From: Circular RNA ZBTB46 depletion alleviates the progression of Atherosclerosis by regulating the ubiquitination and degradation of hnRNPA2B1 via the AKT/mTOR pathway

Fig. 3

CircZBTB46 physically interacts with hnRNPA2B1. (A-C) The relative subcellular localization of circZBTB46 was assessed by qRT‒PCR and FISH. (D) Silver staining of the gel after SDS‒PAGE separation of the proteins that were immunoprecipitated with the 3′ biotin-labeled probe for circZBTB46 (probe) and the biotinylated antisense probe (NC) in RNA pull-down assays. The arrow shows the position of hnRNPA2B1. (E) Mass spectrometry analysis showed that hnRNPA2B1 was the top-ranked protein according to the peptide identification results. (F) Western blot analysis following the RNA pull-down assay and (G) qRT‒PCR analysis following the RIP assay confirmed the interaction between circZBTB46 and hnRNPA2B1. (H) FISH showed that circZBTB46 was colocalized with hnRNPA2B1 in cells and human coronary arteries. (I) 3‑Dimensional structure of the circZBTB46‑hnRNPA2B1 complex

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Immunity & Ageing

ISSN: 1742-4933

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