Fig. 6
Lactate regulates BV2 cell phenotype through lactylation. A-C Expression of IL-1β (A), Arg1 (B), and VEGF (C) at different time points in BV2 cells incubated with Aβ1-42 and Aβ1-42 + NaLA (n = 3 per group, normalized to the untreated sample). D Lactate levels measured at different time points in the conditioned medium of BV2 cells treated with PBS or Aβ1-42(n = 6 per group). E–F Western blot shows the levels of Histone Kla in BV2 cells incubated with Aβ1-42 or Aβ1-42 + NaLA at different time points (n = 3 per group, normalized to expression at 0 h). G Representative images of Pan-Kla immunofluorescence staining. (H-I) Representative western blot and western blot analysis of p300 in BV2 cells (n = 3 per group). J The mRNA expression of p300 (n = 3 per group). K Representative images of Pan Kla western bolts in BV2 cells transfected with con siRNA or p300 si RNA. L-M Expression of related mRNA in BV2 cells transfected with con si RNA or p300 si RNA (n = 3 per group). Date are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Statistical analysis was performed using unpaired Student’s two-tailed t-tests (A-D, F, I-J) or one-way ANOVA, followed by Tukey’s multiple comparisons test (I-M)