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Fig. 2 | Immunity & Ageing

Fig. 2

From: Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly

Fig. 2

Quantification of pro-B and pre-B cells in the bone marrow of HU and aged mice. A, B, C Gates designed to identify pro-B and pre-B cells. These cells were identified based on the expression of the following markers: pro-B (CD19+/B220low/CD43+/IgM-) and pre-B (CD19+/B220low/CD43−/IgM-). A Selection of CD19+ lymphocytes from viable bone marrow cells. B Among CD19+ lymphocytes, cells could be divided into B220 high and B220 low populations. C In the B220 low population, depending on the expression of CD43 and IgM, the pro-B (CD43+/IgM-) and pre-B (CD43-/IgM-) populations could be distinguished. D, E Frequencies of pro-B and pre-B cells in the bone marrow of control (n = 14) vs. HU (n = 13) (D) and young (n = 13) vs. aged (n = 13) (E) mice. Data are shown as the means ± SDs. Statistically significant differences were found using Mann–Whitney or unpaired t tests. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001

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Immunity & Ageing

ISSN: 1742-4933

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