Fig. 4

IGVH segment usage according to their location on the murine IGH locus. A Schematic presentation of distal, median and proximal IGHV segments on the murine IGH locus. B Percentage of IGHV segments belonging to the distal, median and proximal areas in our four groups of mice. C, D, E Dispersion indexes for distal (C), median (D), and proximal (E) IGHV segment usage in control vs. HU and young vs. aged mice. Data are shown as the means ± SDs of 4 groups, each comprising 5 mice (N = 4, n = 5). Statistically significant differences were found using one-way ANOVA followed by a Tukey–Kramer post hoc test. *p ≤ 0.05; ***p ≤ 0.001; n.s., nonsignificant