Fig. 7

Nucleotides trimmed off IGHV, IGHJ and IGHD segments during V(D)J recombination. A, B, C Dispersion indexes of genomic nucleotides deleted at the 3′ end of IGHV (A), at the 5′ end of IGHJ (B), and at both ends of the IGHD segments (indicated by IGHD length) (C) for control vs. HU and young vs. aged mice. D, E Frequencies of sequences according to the length, in nucleotides, of IGHD segments in control vs. HU (D) and young vs. aged (E) mice. Data are shown as the means ± SDs of 4 groups, each comprising 5 mice (N = 4, n = 5). Statistically significant differences were found using one-way ANOVA followed by a Tukey–Kramer post hoc test (A) and Mann–Whitney or unpaired t tests (B). *p ≤ 0.05; n.s., nonsignificant