Fig. 5
From: The effect of metformin on senescence of T lymphocytes
The effect of Metformin on GB secretion. Representative flow histogram of GB secretion by CD3 + CD8 + CD45RA + CCR7-CD27-CD28-CD57 + KLRG1 + (a) and CD3 + CD8 + CD45RA + CCR7-CD27-CD28-CD57-KLRG1- (d) in the control group and the 20 mM Met treatment group relative to the unstimulated controls group from middle-age donors. Analysis of GB production in CD3 + CD8 + CD45RA + CCR7-CD27-CD28-CD57 + KLRG1 + (b) / CD57-KLRG1- (e) T cells, cultured with 0 mM or 20 mM metformin for 24 h in the middle age group. The proportion of CD3 + CD8 + CD45RA + CCR7-CD27-CD28-CD57 + KLRG1 + GB + (c) and CD57-KLRG1-GB + (f) T cells in lymphocytes with metformin treatment compare to control group. g Representative flow histogram of GB secretion by CD3 + CD57 + and CD3 + CD57-T cells relative to the unstimulated controls group in middle-age donors. h Percentage and MFI of GB in CD3 + CD57 + and CD3 + CD57-T cells. Expressed as the mean ± SEM. *P < 0.05; Paired t test. Met, metformin