Fig. 7

Metformin increased the telomerase concentration and the undifferentiated T cells. a The concentration of telomerase in medium (ng/ml) was analyzed in PBMCs with 20 mM Metformin treatment and control at middle age group, examined by ELISA. The flow cytometric analysis the frequency of CD3 + CD4 + CD45RA + CCR7 + CD27 + CD28 + (b) and CD3 + CD8 + CD45RA + CCR7 + CD27 + CD28 + (c) T cells in lymphocytes between the metformin treatment and control. Expressed as the mean ± SEM. *P < 0.05, **P < 0.01; Mann–Whitney test (two-tailed) and paired Student’s t-test. ELISA, Enzyme-linked immunosorbent assay. Met (-), control; Met ( +),20 mM metformin treatment