Fig. 8

The RNA -seq analysis of gene expression associated with T cell senescence with metformin treatment. a Venn diagram portraying the intersections and disjunctive unions of differentially expressed genes in metformin treatment group (green) and control group (gray). b The Volcano Plot showed the overall distribution of genes with up-regulated expression (green), down-regulated expression (dark gray) and no significant difference (light gray) in the metformin treatment group compared to the control group. c Expression heatmap of a selection of senescence- and stemness-associated genes in PBMCs from metformin treatment group and control group at middle-age subjects (n = 3). Data are represented as Z-scores. d Representative genome browser visualizations of normalized reads at indicated gene loci in PBMCs from a representative donor before and after metformin treatment. e The chart portrayed a selection of biological process, from GO functional enrichment analysis, which were up-regulated and down-regulated with metformin treatment. All the above RNA sequencing datas came from three independent donors and three independent experiments