Fig. 3

IL-17A elevated the level of inflammation through the TLR4/NF-κB signalling pathway and microglial activation. A TNF-α mRNA levels in the brain tissue of APP/PS1 mice after injection of IL-17A or IL-17Ab were determined by RT‒qPCR. B TNF-α levels in the brain tissue of APP/PS1 mice after the injection of IL-17A or IL-17Ab were determined by ELISA. C Expression levels of TLR4/NF-κB signalling pathway-related proteins in the brain tissues of APP/PS1 mice that were injected with IL-17A or IL-17Ab. D–F mRNA expression levels of TLR4, NF-κB, and MYD88 in the brain tissue of APP/PS1 mice after the injection of IL-17A or IL-17Ab. G Representative immunofluorescence images of IBA-1⁺ microglia in APP/PS1 mice after the injection of IL-17A or IL-17Ab. The values are expressed as the mean ± standard deviation (****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05). The data are representative of at least three independent experiments