Fig. 4

IL-17A upregulated TNF-α through the TLR4/NF-κB signalling pathway in BV2 cells. A Changes in the TNF-α levels the supernatants of BV2 cells that were stimulated with LPS at different times (ELISA). B Changes in TNF-α mRNA expression in BV2 cells that were incubated with LPS at different times (RT‒qPCR). C TNF-α levels in the supernatants of BV2 cells that were incubated with LPS and IL-17A for 24 h. D TNF-α mRNA levels in BV2 cells that were incubated with LPS and IL-17A for 4 h. E Expression levels of TLR4/NF-κB signalling pathway-related proteins in BV2 cells after IL-17A stimulation for 24 h under inflammatory conditions. F–H mRNA expression levels of TLR4, NF-κB, and MYD88 in BV2 cells that were stimulated with IL-17A for 24 h under inflammatory conditions. The values are expressed as the mean ± standard deviation (****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05). The data are representative of at least three independent experiments