Fig. 1

Partial deletion of SNX27 in CD4-Cre-SNX27^fl/+ mice does not alter lymphoid organ size and cellularity. A-E SNX27^fl/fl or CD4-Cre-SNX27^fl/+ mice were sacrificed. A Expression of SNX27 in thymus and ex vivo-differentiated CTL was examined by Western Blot, confirming partial depletion of SNX27. GAPDH was used as a loading control. A representative blot and the quantification of SNX27 levels in thymus and CTL are shown (n = 6 mice). Values were normalized against GAPDH. Data are shown as mean ± SEM; *p < 0.05; unpaired t-test. B Body weight comparison among age-matched pairs is shown. C Spleens and thymus were extracted and weighted. Scale bar = 1 cm. D Cells from these organs were extracted and total cellularity was analyzed using a cell counter. E Thymocytes were stained for the indicated surface markers and analyzed by flow cytometry (gating strategy shown in Suppl Fig. 1). The percentage of total double positive (DP; CD8⁺CD4⁺) and single positive (SP; CD8⁺CD4⁻ or CD8⁻CD4⁺) thymocytes was calculated (right graph). Representative flow cytometry plots are shown (left panels). Data shown as mean ± SEM of 10–11 mice in (B-D) and 5 mice in (E). Significance in (B) was determined by unpaired t-test, and two-way ANOVA/Bonferroni post-test was used for multiple comparisons in (C-E); ns p > 0.05. CTL Cytotoxic T lymphocyte, SNX Sorting nexin, DP Double positive, SP Single positive, SEM Standard error of the mean