Fig. 5

CD4-Cre-SNX27^fl/+ mice show altered CD4⁺ and CD8⁺ T cell expansion upon activation. A-D Splenocytes from SNX27^fl/fl or CD4-Cre-SNX27^fl/+ mice were stimulated 1, 2, 6 or 7 days with plate-bound anti-CD3 (2.5 µg/ml) and soluble anti-CD28 (1.25 µg/ml). Collected cells were stained for flow cytometry analysis with LIVE/DEAD violet dead cell stain followed by incubation with mouse-directed anti-CD19, anti-CD3, anti-CD4 and anti-CD8 antibodies (gating strategy is shown in Suppl Fig. 4). A Representative density plots from one series of experiments showing the percentage of CD4⁺ and CD8⁺ T cells (CD19⁻ CD3⁺). B Percentage of CD4⁺ cells (CD19⁻ CD3⁺ CD4⁺), (C) CD8⁺ cells (CD19⁻ CD3⁺ CD8⁺), and (D) the ratio between CD8⁺/CD4⁺ T cells is shown. Data shown as mean ± SEM; *p < 0.05; ****p < 0.0001; two-way ANOVA/Bonferroni post-test was used for multiple comparisons; n = 5 mice. SNX Sorting nexin, SEM Standard error of the mean