Fig. 1

Overall effects of aging on the characterization of Hematopoietic Immune system. A Schematic diagram of experimental design for scRNA-seq analysis of bone marrow and spleen cells from 2W and 20M mice group. B UMAP plot showing the immune cell types of hematopoietic Immune system in scRNA-seq. C A heatmap showing scaled expression of discriminative gene sets for each cell type. D Bar chart showing the proportion of immune cell types respectively between the two groups of mice. E UMAP plot showing the immune cell types of the hematopoietic immune system in scRNA-seq respectively between the two groups of mice. F Volcano plot showing the up and downregulated DEGs between the two groups of mice. G Representative GO biological processes and pathways enriched in upregulated DEGs in 20M mice. H Representative GO biological processes and pathways enriched in downregulated DEGs in 20M mice. I Violin plot showing the SASP signaling score between the two groups of mice. J Violin plot showing the SASP signaling score among cell types in 20M mice. Significance in I was calculated using the Wilcoxon test; ****P < 0.0001