Fig. 2

Aging alters the composition and function of T cells in the HIS. A UMAP plot showing the TC subsets of the hematopoietic immune system in scRNA-seq. B A heatmap showing scaled expression of discriminative gene sets for TC subsets. C Pie charts showing the proportion of TC subsets in 2W mice. D Pie charts showing the proportion of TC subsets in 20M mice. E Pseudotime trajectory analysis of CD4 + T cells subsets between two group. Cells are arranged by Pseudotime. F Percentages of CD4 + T cells along the pseudotime for two groups. G Expression transition of Igfbp4, Ccl5, Bhlhe40, Tbc1d4, Stmn1, Foxp3 in CD4 + T cells subsets along the pseudotime. H Representative GO biological process and pathways enriched in upregulated DEGs in CD4 + T cells. I Representative GO biological process and pathways enriched in upregulated DEGs in CD8 + T cells. J Violin plot showing the SASP signaling score between two groups in TC. K Violin plot showing the SASP signaling score among cell types in TC of 20M group. Significance in J was calculated using wilcoxon test; ****P < 0.0001