Fig. 4

The number and functional status of myeloid cells, especially neutrophils, markedly increases with aging. A Volcano plot showing the up or downregulated DEGs in MYE. B Representative GO biological process and pathways enriched in upregulated DEGs in MYE. C The pie chart showing the proportion of MYE subsets between two groups. D Representative GO biological process and pathways enriched in upregulated DEGs among MYE subsets. E Venn diagram showing the interaction of upregulated DEGs and upregulated TFs in MYE. F Ridge plots showing the scores of the Hif1a_targeted_ gene_set among MYE subsets between two groups. G Representative GO biological process and pathways enriched in the intersect of Hif1a_targeted_ gene_set and upregulated DEGs in MYE cells. H The flow cytometry histograms (left) and column charts (right) showing the ration of NEU in bone marrow (up) and spleen (down) (n = 5/group). I The flow cytometry histograms (left) and column charts (right) showing the level of Hif1-α in NEU in bone marrow (up) and spleen (down) (n = 5/group). Significance in H-I was calculated using Student’s t test; *P < 0.05, ***P < 0.001, ****P < 0.0001