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Table 3 Generalized linear mixed models describing the effects of variables on the three defense scores of adults T. molitor that experiences larval grown at different temperature (T20, T24 and T28). The presented models are the most comprehensive ones minimizing the AIC criteria, including either (A) Growth duration or (B) Adult longevity, along with Immune challenge (before vs. after the immune challenge), Age at measurement (hemolymph sampled in young vs. old insects), their two-way and three-way interactions. Body mass of the insects measured before the immune challenge and sex were also included as main effect only. The larval temperature conditions and individual identity were included as random effects. The sample sizes for each category are as follows: T20—40 individuals measured young and 48 old; T24/H85—48 individuals measured young and 37 old; T28—50 individuals measured young and 47 old

From: Growth and longevity modulation through larval environment mediate immunosenescence and immune strategy of Tenebrio molitor

Variables

PC1

PC2

PC3

Wald χ2

d.f

P

Wald χ2

d.f

P

Wald χ2

d.f

P

(A) Models selected with growth duration as variable in the complete model

 Growth

0.016

1

0.9

0.57

1

0.45

6.76

1

0.009

 Challenge

16.1

1

 < 0.001

53.1

1

 < 0.001

1161

1

 < 0.001

 Age

1.48

1

0.22

-

-

-

14.9

1

 < 0.001

 Mass

8.8

1

0.003

2.88

1

0.09

-

-

-

 Sex

-

-

-

-

-

-

-

-

-

 Growth*Challenge

3.08

1

0.08

5.29

1

0.02

-

-

-

 Growth*Age

-

-

-

-

-

-

-

-

-

 Challenge*Age

-

-

-

-

-

-

8.99

1

0.003

 Growth*Challenge*Age

20.4

2

 < 0.001

-

-

-

22.6

3

 < 0.001

(B) Models selected with adult longevity as variable in the complete model

 Longevity

1.73

1

0.19

4.55

1

0.03

5.01

1

0.03

 Challenge

16.2

1

 < 0.001

55.1

1

 < 0.001

1116

1

 < 0.001

 Age

1.88

1

0.17

-

-

-

17.0

1

 < 0.001

 Mass

20.2

1

 < 0.001

4.31

1

0.04

-

-

-

 Sex

-

-

-

-

-

-

-

-

-

 Longevity*Challenge

-

-

-

3.13

1

0.077

-

-

-

 Longevity*Age

-

-

-

-

-

-

-

-

-

 Challenge*Age

1.14

1

0.29

-

-

-

8.62

1

0.003

 Longevity*Challenge*Age

-

-

-

-

-

-

6.76

3

0.08