Fig. 2

Age-associated decline in ovarian function correlates with elevated expression of inflammatory factors. A Visualization of TUNEL-labeled apoptotic cells (in green) with DAPI counterstaining (in blue) in the ovaries of mice at 3 months (3 m), 6 months (6 m), and 9 months (9 m) of age. B Comprehensive data on body weight, ovary weight, and ovarian index for each group of mice (n = 12 for each group). C Representative histological images obtained through H&E staining of murine ovarian tissue sections from each experimental group (at 200 × magnification). Atretic follicles are indicated by black arrows. D Quantitative analysis of the average number of follicles per tissue section for each experimental group (n = 5 for each group). E Assessment of relative mRNA expression levels for genes associated with inflammation and senescence-associated secretory phenotype (SASP) (n = 6 for each group). *P < 0.05, **P < 0.01, ***P < 0.001