Fig. 4
From: Chitosan alleviates ovarian aging by enhancing macrophage phagocyte-mediated tissue homeostasis
The ovarian immune milieu is altered with age. A Analysis of immune cell infiltration between ovarian tissues from mice at 9 months (9 m) and 3 months (3 m) of age (Reproduction, 2020). B Immunofluorescence staining of F4/80 in sections of ovarian tissue from mice. C The change of ovarian macrophages polarization over the course of aging. Percentage and number of M1 (CD86 +) and M2 (CD163 +) macrophage from the ovaries of mice at 3 months (3 m), 6 months (6 m), 9 months (9 m) of age were analyzed using flow cytometry (n = 5 for each group). The bar charts reflect the percentage of M1 (CD86 +) and M2 (CD163 +) macrophage within total ovarian macrophages. *P < 0.05, **P < 0.01, ***P < 0.001