Fig. 3

Differences in cytokines and CD8 T-cell responses on day 7 post stimulation. PBMCs stimulated with irradiated SARS-CoV-2 (IRR) were cultured for 7 days. The collected cells were stained for CD8, and granzyme B. The supernatant was quantitated for cytokines by multiplex. (a) IFN-α; (b) IL-29. Dot plot depicts the (c) % of viable CD8 T cells; (d) % of CTLs obtained using flow cytometry. (e) IFN-γ. Mean + S.E. Young = 20; Mid-age-20; Aged = 20 subjects. The P value between the control and SARS-CoV-2-stimulated conditions in the three age groups was calculated using the parametric paired t test. Significance between different age groups was calculated using ordinary one way ANOVA followed by Tukey’s test