Fig. 4

Transcriptomic analysis of unstimulated PBMCs of different age groups. We performed total RNA-seq analyses of unstimulated PBMCs from young (Y), middle-aged (M) and aged (A) individuals. (a) Venn diagram depicting the number of common and unique genes changed in A vs. Y, A vs. M and M vs. Y. Pathway analysis was done using Ingenuity pathway analysis (IPA) software. Bar graphs depicting selected up- and downregulated pathways for (b) A vs. Y upregulated; (c) A vs. Y downregulated; (d) A vs. M upregulated; (e) A vs. M downregulated; (f) M vs. Y upregulated; (g) M vs. Y downregulated. Heatmaps show selected (h) upregulated and (i) downregulated pathways after comparative pathway analysis of the three age groups. N = A-4; M-9; Y-4. Fisher’s Exact Test is used by IPA to calculate significance in pathway analysis including comparative pathway analysis. Values below 1.3 are not significant