Table 8 Designed primer sequences for TBX21 and GAPDH genes
Gene | Primer | Sequence (5’ > 3’) | Length (nt) | GC (%) | Tm (°C) | Product size (bp) |
|---|---|---|---|---|---|---|
TBX21 NM_013351.2 | Forward | 5- GTGACCCAGATGATTGTGCTCC -3 | 22 | 54.55 | 61.26 | 114 |
Revers | 5- GATATGCGTGTTGGAAGCGTTG -3 | 22 | 50.00 | 60.79 | ||
GAPDH NM_002046.7 | Forward | 5-CATCACCATCTTCCAGGAGCG-3 | 21 | 57.14 | 60.81 | 235 |
Revers | 5-GGAGGCATTGCTGATGATCTTG-3 | 22 | 50.00 | 59.71 |