Skip to main content
Download PDF

Associations between circulating cell-free mitochondrial DNA, inflammatory markers, and cognitive and physical outcomes in community dwelling older adults

Immunity & Ageing volume 20, Article number: 24 (2023) Cite this article

Abstract

Background

Dementia and frailty are common age-related syndromes often linked to chronic inflammation. Identifying the biological factors and pathways that contribute to chronic inflammation is crucial for developing new therapeutic targets. Circulating cell-free mitochondrial DNA (ccf-mtDNA) has been proposed as an immune stimulator and potential predictor of mortality in acute illnesses. Dementia and frailty are both associated with mitochondrial dysfunction, impaired cellular energetics, and cell death. The size and abundance of ccf-mtDNA fragments may indicate the mechanism of cell death: long fragments typically result from necrosis, while short fragments arise from apoptosis. We hypothesize that increased levels of necrosis-associated long ccf-mtDNA fragments and inflammatory markers in serum are linked to declines in cognitive and physical function, as well as increased mortality risk.

Results

Our study of 672 community-dwelling older adults revealed that inflammatory markers (C-Reactive Protein, soluble tumor necrosis factor alpha, tumor necrosis factor alpha receptor 1 [sTNFR1], and interleukin-6 [IL-6]) positively correlated with ccf-mtDNA levels in serum. Although cross-sectional analysis revealed no significant associations between short and long ccf-mtDNA fragments, longitudinal analysis demonstrated a connection between higher long ccf-mtDNA fragments (necrosis-associated) and worsening composite gait scores over time. Additionally, increased mortality risk was observed only in individuals with elevated sTNFR1 levels.

Conclusion

In a community dwelling cohort of older adults, there are cross-sectional and longitudinal associations between ccf-mtDNA and sTNFR1 with impaired physical and cognitive function and increased hazard of death. This work suggests a role for long ccf-mtDNA as a blood-based marker predictive of future physical decline.

Background

The rapid advancements in technology and medicine over the past century have led to significant increases in human lifespan. As a result, we have also seen a rise in the occurrence of age-related syndromes, such as Alzheimer’s disease (AD), frailty, and sarcopenia [1]. As people grow older, the likelihood of developing these conditions increases; a 2020 study found that 41% of older adults screened positive for frailty, while 28% screened positive for AD [1].

While increasing age is a major risk factor for AD, frailty, a geriatric syndrome characterized by reduced resilience to physical and psychosocial stressors, also has a significant impact on AD progression [2,3,4]. A recent study conducted using the UK Biobank data discovered a strong association between frailty and the development of AD; frail individuals, on average, developed AD 3.6 years earlier than their non-frail counterparts [2].

Given the profound connection between age-related cognitive and physical decline, it is crucial to investigate the common biological factors contributing to the progression of geriatric syndromes, including AD and frailty. By doing so, we can better identify those at high risk of developing these conditions and work towards improved prevention and treatment strategies.

A commonly observed feature in both AD and frailty is the chronically low-grade elevation in pro-inflammatory markers such as interleukin-6 (IL-6), interleukin-1β (IL-1β), C-reactive protein (CRP), and tumor necrosis factor alpha (TNF⍺) [5,6,7]. Numerous studies have examined inflammatory marker elevations in both blood and cerebrospinal fluid of individuals with preclinical AD and a subset of them show promise as an easy to measure biomarker indicating disease risk and progression [8,9,10]. Elevated plasma soluble tumor necrosis factor alpha receptor 1 (sTNFR1) has shown associations with progression to mild cognitive impairment in cognitively healthy individuals [10]. Despite these associations with inflammation, the biological mechanisms underlying the elevations in these pathways remain poorly understood.

A potential mediator of chronic inflammation in both cognitive and physical decline is circulating cell-free DNA (ccf-DNA), specifically ccf-DNA originating from mitochondrial DNA sources (ccf-mtDNA). Mitochondrial DNA can trigger innate immune system DNA sensors, and its presence acts as damage associated molecular patterns (DAMPs), which are intracellular components released in the setting of tissue and cell injury. In addition to functioning as a DAMP, ccf-mtDNA can mimic some of the characteristics of bacterial and viral nucleic acids. Mitochondrial DNA demonstrates different methylation patterns and hypomethylation of CpG regions relative to genomic DNA, which allow it to be recognized as non-self, further contributing to immune activation [11].

Cell death processes lead to the generation of different ccf-mtDNA sizes. Apoptosis leads to shorter DNA fragmentation patterns (short ccf-mtDNA), while necrosis typically results in variable, less-regulated DNA fragmentation, leading to long ccf-mtDNA fragments [12]. Necrosis, along with other inflammatory cell death processes like pyroptosis, results in the release of cell contents, which generate various immune-stimulating DAMPs [13]. It is plausible that this release of DAMPs could contribute to a persistent low-grade inflammation linked to age-related physical and cognitive decline [14, 15].

In addition to cell death, cell homeostatic mechanisms such as mitophagy are also associated with release of mtDNA into circulation [16]. Mitophagy and the related cell homeostasis process of autophagy have been well studied as a mechanism that is disrupted in aging and neurodegenerative diseases, leading to impaired energy utilization and cellular dysfunction [17]. With a variety of mechanisms resulting in ccf-mtDNA release, prior studies have demonstrated associations between cell necrosis and apoptosis with fragment size. Therefore, quantifying relative sizes of ccf-mtDNA can lead to better understanding of different types of cell turnover processes in aging adults.

Prior studies demonstrate associations between higher levels of plasma ccf-mtDNA with increased mortality and morbidity in several acute diseases [18,19,20]. Additionally, elevated ccf-mtDNA is associated with poorly controlled chronic diseases that are characterized by cellular stress, including atrial fibrillation, type 2 diabetes and major depressive disorder [21,22,23]. Older adults with both depression and frailty demonstrate even higher levels of ccf-mtDNA than individuals with either condition alone [24]. Prior studies in cerebrospinal fluid have showed varying trends with respect to levels of ccf-mtDNA in AD [25, 26]. However, additional studies with larger sample sizes are needed to examine associations between cognitive decline and ccf-mtDNA in peripheral blood, and their relationships with previously identified peripheral inflammatory markers (IL-6, CRP, and TNF⍺).

The goal of this study is to identify relationships of serum ccf-mtDNA and inflammatory markers with longitudinal cognitive and physical outcomes in a pooled sample of three community-based cohorts of older adults. We hypothesize that ccf-mtDNA, specifically necrosis-associated long ccf-mtDNA fragments are associated with adverse cognitive and physical outcomes, both cross-sectionally and longitudinally.

Results

The populations used in this study are three longitudinal clinical-pathologic cohort studies of aging and Alzheimer’s disease based at the Rush Alzheimer’s Disease Center: Religious Orders Study (ROS); Memory and Aging Project (MAP); and Minority Aging Research Study (MARS). Descriptive statistics of the study population are in Table 1. Mean age of participants at baseline was 80 years old and participants were predominantly female, white, and completed post-secondary education. Mean concentrations of short ccf-mtDNA and long ccf-mtDNA were 390 copies/µl and 160 copies/µl, respectively (Table 1). Box plots with the distribution of untransformed ccf-mtDNA values are provided as Supplementary Fig. 1. Mean concentrations of inflammatory markers for individuals at baseline were as follows: CRP = 1.3 (± 0.8) µg/ml; sTNFR1 = 7.4 (± 0.4) pg/ml; TNF⍺ = 1.5 (± 0.6) pg/ml; and IL-6 = 0.9 (± 0.6) pg/ml (Table 1). Percent of individuals with missing data included: global cognition (9.2%); frailty (18%); grip strength (38.5%); gait speed (19.3%); motor gait (14.3%); motor composite score (16.4%). Twenty-seven participants from the starting 672 individuals died over the course of this study.

Table 1 Descriptive data of participants at baseline (N = 672) a
Full size table

Cross-sectional analyses

Given that ccf-mtDNA can activate the immune system [27], we examined cross-sectional associations between ccf-mtDNA and inflammatory markers in serum. We found significant associations between short ccf-mtDNA fragments and all four inflammatory markers (CRP, β = 0.47 (CI 0.25, 0.69); TNF⍺, β = 0.15 (CI 0.07, 0.23); sTNFR1, β = 0.51 (CI 0.33, 0.70); IL-6, β = 0.41 (CI 0.26, 0.56)), as well as significant associations between long ccf-mtDNA fragments and CRP, TNF⍺, and IL-6 (Table 2). There was no association between long ccf-mtDNA and sTNFR1.

Table 2 Associations of ccf-mtDNA with inflammatory markers (N = 672)
Full size table

We next identified associations between ccf-mtDNA and cognitive and physical outcomes measured in the ROS-MAP and MARS study, specifically global cognition and scores for cognitive domains as well as physical functioning measures including frailty, grip strength, gait speed and composite motor scores. No significant cross-sectional associations were found between ccf-mtDNA and cognitive and physical function when adjusting for inflammatory markers (Table 3).

Table 3 Associations of ccf-mtDNA with cognitive and physical outcomes adjusting for inflammatory biomarkers (N = 672)
Full size table

Prior studies have shown associations between frailty and cognition with elevated inflammatory cytokines (IL-6 and sTNFR1) [10, 28]. Therefore, we next examined cross-sectional associations between inflammatory markers and physical and cognitive outcomes in our cohort, and saw associations with lower grip strength, reduced gait speed, and increased frailty scores (Table 4). In particular, soluble TNFR1 demonstrated statistically significant associations with all cognitive and physical measures in our study and CRP also demonstrated significant associations with cognitive and physical measures. Elevated sTNFR1 levels were associated with lower cognition scores in all categories, and worse physical function measures. CRP was significantly associated with semantic memory, perceptual speed and perceptual orientation and the composite measures of motor gait and motor composite score. Elevated levels of IL-6 was only associated with reduced motor composite score.

Table 4 Associations of peripheral inflammatory markers with cognitive and physical outcomes (N = 672)
Full size table

Longitudinal analyses

Longitudinal associations between ccf-mtDNA fragments, frailty and global cognitive scores were next examined using random effects modeling over an eight year follow up period. Higher levels of long ccf-mtDNA were associated with steeper declines (worsening) in the motor gait score (walking speed and turning) slopes over time (Table 5).

Table 5 Adjusted longitudinal changes in global cognition score and physical function relative to baseline ccf-mtDNA levels (N = 672)
Full size table

Given the strong cross-sectional associations between inflammatory markers and declining physical function, we next examined associations between ccf-mtDNA and inflammatory markers with mortality in this cohort. When adjusting for demographics, higher levels of short ccf-mtDNA was associated with elevated hazard of death (HR = 3.54, CI 1.02–12.30), although this significance did not remain when adjusting for inflammatory variables (Table 6, Model 2). In survival analyses with 4,921.5 person-years, elevated levels of sTNFR1 were associated with a higher hazard of death over an 8-year follow up period when adjusting for demographics (HR = 4.37, CI 1.19–16.00). Long ccf-mtDNA did not have associations with mortality (Table 6, Model 3).

Table 6 Associations of ccf-mtDNA and peripheral inflammatory markers with mortality (N = 672)
Full size table

Discussion

This study shows cross-sectional and longitudinal relationships between peripheral inflammatory markers (ccf-mtDNA and sTNFR1), and cognitive and physical decline in a community dwelling population of older adults. Interestingly, short and long ccf-mtDNA were positively associated with most inflammatory markers measured at the same time point, with the exception of no significant association between long ccf-mtDNA and sTNFR1. Additionally, there were no associations between ccf-mtDNA fragments and cognitive and physical outcomes on cross-sectional analysis, but high levels of long ccf-mtDNA fragments were associated with worsening motor gait scores over an eight-year period. Short ccf-mtDNA fragments and sTNFR1were associated with higher hazard of death over an eight year follow up period, but this association went away when adjusting for inflammatory markers.

Compared to prior analysis of genomic-derived ccf-DNA in the ROS-MAP cohort [29], we did not observe cross-sectional associations between ccf-mtDNA fragments and cognitive and physical outcomes. However, longitudinal analysis demonstrated associations with a composite gait measure for the long ccf-mtDNA fragment after adjusting for inflammatory markers (Table 5), suggesting that mitochondrial derived circulating cell-free DNA may be more strongly linked to physical decline, while genomic derived ccf-DNA is linked to both cognitive and physical decline.

We believe that several factors could be contributing to these findings. Firstly, ccf-mtDNA may be degrading too quickly to be accurately detected in our banked serum samples. Previous studies have discussed degradation of circulating longer fragment ccf-mtDNA as a factor contributing to higher levels of detectable smaller ccf-mtDNA fragments in circulation [12, 30].

Secondly, we may find more associations with ccf-mtDNA and physical and cognitive decline when looking at either a younger population or a population with a higher percentage of males. Our study population, though well-characterized, is primarily female, and the mean age is 80. By examining middle-aged and older adults in a more balanced gender distribution, more connections may be uncovered between age-related decline and long ccf-mtDNA levels, both cross-sectionally and longitudinally.

We tested whether mean levels of ccf-mtDNA differ by whether cognitive and physical outcomes are missing using t-tests. We detected no differences for global cognitive function, working memory, semantic memory, perceptual speed, perceptual orientation, episodic memory, and grip strength. Interestingly, we observed in our study population that individuals missing frailty measurements had higher levels of ccf-mtDNA at that visit. The frailty score through the ROS-MAP and MARS studies is a composite of 5 individual tests, two of which require the participant to perform physical tests at the study site. We believe these associations between high ccf-mtDNA and missing frailty scores could be due to individuals with significant functional limitation who were not able to complete the physical tests at the visit. This is also a likely factor contributing to the higher percentage of missing data for gait speed, frailty, and grip strength when compared to the percent missing data for global cognition.

A distinguishing characteristic of our study compared to previous work examining associations between ccf-mtDNA and mortality is that the ROS-MAP and MARS populations includes community dwelling older adults, while other studies were in hospitalized, acutely ill populations [20, 31]. Despite this major difference in the participants sampled and different methods of ccf-mtDNA isolation and measurement, we observed similar associations between elevations in ccf-mtDNA and increased mortality in our study. Increases in circulating DAMPs (ccf-mtDNA) may be contributing to activation of inflammatory pathways which subsequently leads to increased mortality.

An additional consideration with our study is the potential for cellular mitochondrial DNA contamination that could be a result of varying sample processing over the course of the ROS-MAP study. To address these concerns, future studies can use specialized blood collection tubes for cell-free DNA analysis, minimizing this effect.

Soluble TNFR1 has been identified as an inflammatory marker predictive of frailty and increased mortality in other studies [28], and our study reaffirms these findings as well as identifies associations between elevated sTNFR1 and lower cognitive test scores (both global cognitive score and cognitive subdomain scores) [10, 32]. Interestingly, our cross-sectional analysis of inflammatory markers and cognitive testing showed no associations between IL-6 and these cognitive domains, in contrast to other studies [10, 33], and this could be due in part to demographic differences in the study populations. The other studies focused on younger adults (mean age 60) compared to this study (mean age 80), while others had more even distributions of men/women compared to this study (81% female), and others had higher percentages of smokers. Additionally, differences in ELISA assays for quantifying the inflammatory markers could have contributed to the differences in mean cytokine values at baseline in these other studies compared to ours.

A question that remains is whether increases in ccf-mtDNA precede the elevations in inflammatory markers observed in individuals with frailty and cognitive decline, or if the cell death releasing ccf-mtDNA in circulation occurs because of increased inflammation. Follow up studies will also examine the associations between ccf-mtDNA levels with AD biomarkers including amyloid-beta and tau proteins. These questions can be further studied in human cell lines and mouse models of AD.

Studies using human serum at this time are not able to convey information about cellular processes such as mitophagy, that can also contribute to ccf-mtDNA levels. Nonetheless, differentiating between mitophagy and necrosis/apoptosis is crucial for a deeper comprehension of how these processes alter with aging and contribute to the generation of ccf-mtDNA. Additionally, aging studies using animal models with targeted genetic deletions in mitophagy genes can also be used to understand the degree to which mitophagy and autophagy contributes to ccf-mtDNA versus apoptosis or necrosis.

Ccf-mtDNA are released passively and actively into circulation from a wide variety of cell death processes and are known to be immunostimulatory by activating the innate immune system through DNA sensing pathways [11]. Little is known about aging-related changes in these immune pathways, particularly changes in the expression of DNA sensors in peripheral immune cells and the brain of individuals with elevated risk of dementia, which can possibly lead to increased inflammatory activation. Future studies can focus on elucidating this relationship and the role of immunostimulatory ccf-mtDNA in contributing to chronic inflammation. Ultimately, better characterization of the downstream effects of ccf-mtDNA and sTNFR1 on chronic inflammation in AD and frailty can help identify novel drug targets that can reduce the high mortality and morbidity of these syndromes.

Conclusions

This study identifies serum ccf-mtDNA as a promising biomarker linked to chronic inflammation, which could serve as an indicator for individuals at a heightened risk of physical decline and mortality. Furthermore, we underscore the importance of sTNFR1, which demonstrates a strong connection with cognitive and physical deterioration, an increased likelihood of death, and a relationship with apoptosis-associated short ccf-mtDNA fragment. Future studies will include mechanistic studies to better understand the relationships between ccf-mtDNA fragments and chronic inflammation, particularly how these elements interact with pathways known to be disrupted cognitive and physical decline, thereby enhancing our comprehension of these intricate relationships.

Methods

Study sample

Data were obtained from the Rush Alzheimer’s Disease Center Religious Orders Study, Rush Memory and Aging Project (ROS-MAP) and Minority Aging Research Study (MARS), which have been detailed in prior publications [34, 35]. ROS began in 1994 and consists of nuns, priests, and brothers from across the USA, MAP began in 1997 and consists of community-dwelling older adults from the greater Chicago metropolitan area, and MARS began in 2004 and consists of community-dwelling older African Americans free of dementia. All studies were approved by the Institutional Review Board at Rush University Medical Center. All participants signed an informed consent, Anatomic Gift Act, and a repository consent to allow their resources to be shared. Annual assessments are conducted for all participants. Serum samples were obtained from ROS-MAP and MARS participants during cohort evaluations; we included here 672 samples which were available for analysis at the time of this study.

Biological measures

Digital PCR: The digital PCR method was used to directly quantify mitochondrial-derived ccf-DNA. Two sets of primers were designed targeting a conserved region of the mitochondrial ribosomal 16 S gene (Integrated DNA Technologies, Iowa City, IA); the first primer pair amplified a 79-bp fragment (short ccf-mtDNA, apoptotic fragment) and the second primer pair amplified an adjacent 230-bp fragment (long ccf-mtDNA, necrotic fragment).

Run preparation: Serum was diluted with PBS and heat denatured, then vortexed until the solid pellet broke apart, approximately 3 s. The samples were then spun down in a microcentrifuge for 15 s to pellet any viscous material. 10 µl of the supernatant was mixed with 10 µl of water, vortexed and spun down. One Master mix contained two assays: “long fragment” labeled with Cy5, and “short fragment” labeled with Hex. 16.4 µl of Master mix was mixed with 4 µl of the prepared serum and the entire volume was loaded into the digital PCR plate and run on the Constellation digital PCR system (Formulatrix, Bedford, MA) using the following thermocycling conditions: [98 C for 120 s, 40 cycles of {98 C for 10 s, 60 C for 30 s}, 45 C for 30 s].

Data calling: Amplification thresholds were applied using the Constellation software (Formulatrix, Bedford MA). Results were exported and scripts developed by Formulatrix were run to distinguish between partitions which had amplification with both mitochondrial assays and those which amplified only one.

ELISA: Peripheral inflammatory markers C-reactive protein, sTNFR1, TNF⍺, and IL-6 were assayed in serum from the RADC biorepository. All assays were performed on the Meso Scale Discovery (MSD) V-plex electrochemiluminescence platform at the Research Laboratory Core of the Institute for Clinical and Translational Research at Johns Hopkins University. Interassay coefficients of variation using controls on every plate were as follows: CRP = 8.46%; sTNFR1 = 7.44%; TNF⍺/IL-6 = 5.12%. Inflammatory markers were log transformed and generalized estimating equation (GEE) models were used to determine the relationship between cytokines and cognition and physical function outcomes.

Cognitive measures

Twenty-one cognitive tests were conducted at each study visit, nineteen of which were used to assess the following cognitive domains: episodic memory, semantic memory, working memory, perceptual orientation, and perceptual speed. Each test had a z-score constructed using all participants and each cognitive domain z-score was calculated by averaging all z-scores within that domain. A global cognitive function t-score was calculated by averaging the z-score of each of the 19 cognitive tests [36].

A three-stage process consisting of computer scoring of cognitive tests, clinical judgement by a neuropsychologist and diagnostic classification by a clinician was used to assess dementia status at each study visit [37, 38]. Additional details on diagnostic criteria for this study is detailed in Nidadavolu et al. [29].

Physical measures

Physical measures (grip strength, gait speed, body composition, and fatigue, global motor function composite score) were obtained at each study visit to create a frailty composite z-score. Methodologic details are in Nidadavolu et al. [29].

Adjustment variables

Race in the ROS-MAP and MARS studies was self-reported as White, Black or African American, American Indian or Alaska Native, Native Hawaiian or Other Pacific Islander, Asian, Other, or Unknown. A binary race variable was defined as 0 = White and 1 = any other race; this binary race classification was used in all analyses since there were few participants who reported race other than White or African American. Analyses were adjusted for age, sex, race, and years of education.

Analyses

We only used complete cases for both predictor and outcome variables in this study. We described the sample initially with descriptive statistics. Generalized estimating equations (GEE) were used to estimate the cross-sectional associations between the log-transformed numbers of copies of short and long ccf-mtDNA fragments with inflammatory markers (i.e. CRP, sTNFR1, TNF⍺, IL-6). Because of the highly skewed nature of inflammatory markers, we log transformed values of all the above-mentioned markers. Similarly, GEE models were used to estimate cross-sectional associations between ccf-mtDNA predictors (i.e. log-transformed numbers of copies of short and long ccf-mtDNA fragments) with cognitive measures (i.e. global cognition, episodic memory, semantic memory, working memory, perceptual speed, and perceptual orientation), as well as frailty and other physical functioning outcomes (grip strength, gait speed, motor composite score). Another set of Generalized estimating equations (GEE) was used to estimate the association between inflammatory markers and cognitive and physical outcomes. Longitudinal associations between ccf-mtDNA and changes in cognitive (global, episodic memory, semantic memory, working memory, perceptual speed, and perceptual orientation) and physical outcomes (frailty, gait speed, grip strength, hand strength, and motor composite score) were evaluated using linear latent growth model, which is analogous to mixed effects models with random effects for participants and time [39]. We then ran separate Cox proportional-hazards models to estimate hazard ratios for mortality, with each ccf-mtDNA, cytokines and physical and cognitive measures as the main predictor. All models were adjusted for baseline age, sex, race, and education. Stata version 17.0 was used for analysis. This study was approved by the Johns Hopkins University and Rush University Medical Center Institutional Review Boards (IRB).

Data Availability

Data are available upon reasonable request to the corresponding authors.

Abbreviations

AD:

Alzheimer’s disease

ccf-mtDNA:

Circulating cell-free mitochondrial DNA

CI:

Confidence Interval

CRP:

C-reactive protein

DAMP:

Damage associated molecular patterns

ELISA:

Enzyme-linked immunoassay

GEE:

Generalized estimating equations

IL-6:

Interleukin-6

MAP:

Memory and Aging Project

MARS:

Minority Aging Research Study

ROS:

Religious Orders Study

sTNFR1:

Soluble tumor necrosis factor ⍺ receptor 1

TNF⍺:

Tumor necrosis factor ⍺

References

  1. Sanford AM, Morley JE, Berg-Weger M, Lundy J, Little MO, Leonard K, et al. High prevalence of geriatric syndromes in older adults. PLoS ONE. 2020;15:e0233857.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Petermann-Rocha F, Lyall DM, Gray SR, Esteban-Cornejo I, Quinn TJ, Ho FK, et al. Associations between physical frailty and dementia incidence: a prospective study from UK Biobank. Lancet Healthy Longev Elsevier. 2020;1:e58–68.

    Article  Google Scholar 

  3. Wallace LMK, Theou O, Godin J, Ward DD, Andrew MK, Bennett DA, et al. 10-year frailty trajectory is associated with Alzheimer’s dementia after considering neuropathological burden. Aging Med Milton NSW. 2021;4:250–6.

    Article  Google Scholar 

  4. Kulmala J, Nykänen I, Mänty M, Hartikainen S. Association between frailty and dementia: a population-based study. Gerontology. 2014;60:16–21.

    Article  PubMed  Google Scholar 

  5. Basu A, Krady JK, Levison SW. Interleukin-1: a master regulator of neuroinflammation. J Neurosci Res. 2004;78:151–6.

    Article  CAS  PubMed  Google Scholar 

  6. Landino K, Tanaka T, Fantoni G, Candia J, Bandinelli S, Ferrucci L. Characterization of the plasma proteomic profile of frailty phenotype. GeroScience. 2021;43:1029–37.

    Article  CAS  PubMed  Google Scholar 

  7. Walker KA, Gottesman RF, Wu A, Knopman DS, Gross AL, Mosley TH, et al. Systemic inflammation during midlife and cognitive change over 20 years: the ARIC Study. Neurology. 2019;92:e1256–67.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Brosseron F, Krauthausen M, Kummer M, Heneka MT. Body fluid cytokine levels in mild cognitive impairment and Alzheimer’s disease: a comparative overview. Mol Neurobiol. 2014;50:534–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gross AL, Xue Q-L, Bandeen-Roche K, Fried LP, Varadhan R, McAdams-DeMarco MA, et al. Declines and impairment in executive function predict onset of physical Frailty. J Gerontol A Biol Sci Med Sci. 2016;71:1624–30.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Gross AL, Walker KA, Moghekar AR, Pettigrew C, Soldan A, Albert MS, et al. Plasma markers of inflammation linked to clinical progression and decline during preclinical AD. Front Aging Neurosci. 2019;11:229.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. West AP, Khoury-Hanold W, Staron M, Tal MC, Pineda CM, Lang SM, et al. Mitochondrial DNA stress primes the antiviral innate immune response. Nature. 2015;520:553–7.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Budnik LT, Kloth S, Baur X, Preisser AM, Schwarzenbach H. Circulating mitochondrial DNA as Biomarker linking Environmental Chemical exposure to early preclinical lesions elevation of mtDNA in human serum after exposure to carcinogenic halo-alkane-based pesticides. PLoS ONE. 2013;8:e64413.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Frank D, Vince JE. Pyroptosis versus necroptosis: similarities, differences, and crosstalk. Cell Death Differ. 2019;26:99–114.

    Article  PubMed  Google Scholar 

  14. Ferrucci L, Fabbri E. Inflammageing: chronic inflammation in ageing, cardiovascular disease, and frailty. Nat Rev Cardiol. 2018;15:505–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Mukhara D, Oh U, Neigh GN, Neuroinflammation. Handb Clin Neurol. 2020;175:235–59.

    Article  PubMed  Google Scholar 

  16. Jing R, Hu Z-K, Lin F, He S, Zhang S-S, Ge W-Y, et al. Mitophagy-mediated mtDNA release aggravates Stretching-Induced inflammation and lung epithelial cell Injury via the TLR9/MyD88/NF-κB pathway. Front Cell Dev Biol. 2020;8:819.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Tran M, Reddy PH. Defective autophagy and Mitophagy in Aging and Alzheimer’s Disease. Front Neurosci. 2020;14:612757.

    Article  PubMed  Google Scholar 

  18. Faust HE, Reilly JP, Anderson BJ, Ittner CAG, Forker CM, Zhang P, et al. Plasma mitochondrial DNA levels are Associated with ARDS in Trauma and Sepsis Patients. Chest. 2020;157:67–76.

    Article  CAS  PubMed  Google Scholar 

  19. Nakahira K, Kyung S-Y, Rogers AJ, Gazourian L, Youn S, Massaro AF, et al. Circulating mitochondrial DNA in patients in the ICU as a marker of mortality: derivation and validation. PLoS Med. 2013;10:e1001577. discussion e1001577.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Scozzi D, Cano M, Ma L, Zhou D, Zhu JH, O’Halloran JA, et al. Circulating mitochondrial DNA is an early indicator of severe illness and mortality from COVID-19. JCI Insight. 2021;6:143299.

    PubMed  Google Scholar 

  21. Lindqvist D, Wolkowitz OM, Picard M, Ohlsson L, Bersani FS, Fernström J, et al. Circulating cell-free mitochondrial DNA, but not leukocyte mitochondrial DNA copy number, is elevated in major depressive disorder. Neuropsychopharmacol Off Publ Am Coll Neuropsychopharmacol. 2018;43:1557–64.

    Article  CAS  Google Scholar 

  22. Liu J, Cai X, Xie L, Tang Y, Cheng J, Wang J, et al. Circulating cell free mitochondrial DNA is a biomarker in the development of Coronary Heart Disease in the patients with type 2 diabetes. Clin Lab. 2015;61:661–7.

    Article  CAS  PubMed  Google Scholar 

  23. Wiersma M, van Marion DMS, Bouman EJ, Li J, Zhang D, Ramos KS, et al. Cell-free circulating mitochondrial DNA: a potential blood-based marker for Atrial Fibrillation. Cells. 2020;9:E1159.

    Article  Google Scholar 

  24. Ampo E, Mendes-Silva AP, Goncalves V, Bartley JM, Kuchel GA, Diniz BS. Increased levels of circulating cell-free mtDNA in the plasma of subjects with late-life Depression and Frailty: a preliminary study. Am J Geriatr Psychiatry Off J Am Assoc Geriatr Psychiatry. 2022;30:332–7.

    Article  Google Scholar 

  25. Cervera-Carles L, Alcolea D, Estanga A, Ecay-Torres M, Izagirre A, Clerigué M, et al. Cerebrospinal fluid mitochondrial DNA in the Alzheimer’s disease continuum. Neurobiol Aging. 2017;53:192e1–4.

    Article  Google Scholar 

  26. Podlesniy P, Figueiro-Silva J, Llado A, Antonell A, Sanchez-Valle R, Alcolea D, et al. Low cerebrospinal fluid concentration of mitochondrial DNA in preclinical Alzheimer disease. Ann Neurol. 2013;74:655–68.

    Article  CAS  PubMed  Google Scholar 

  27. Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, et al. Circulating mitochondrial DNA increases with age and is a familiar trait: implications for “inflamm-aging. Eur J Immunol. 2014;44:1552–62.

    Article  CAS  PubMed  Google Scholar 

  28. Van Epps P, Oswald D, Higgins PA, Hornick TR, Aung H, Banks RE, et al. Frailty has a stronger association with inflammation than age in older veterans. Immun Ageing A. 2016;13:27.

    Article  Google Scholar 

  29. Nidadavolu LS, Feger D, Wu Y, Grodstein F, Gross AL, Bennett DA, et al. Circulating cell-free genomic DNA is Associated with an increased risk of Dementia and with change in cognitive and physical function. J Alzheimers Dis JAD. 2022;89:1233–40.

    Article  CAS  PubMed  Google Scholar 

  30. Kustanovich A, Schwartz R, Peretz T, Grinshpun A. Life and death of circulating cell-free DNA. Cancer Biol Ther. 2019;20:1057–67.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Kayhanian S, Glynos A, Mair R, Lakatos A, Hutchinson PJA, Helmy AE, et al. Cell-free mitochondrial DNA in Acute Brain Injury. Neurotrauma Rep. 2022;3:415–20.

    Article  PubMed  PubMed Central  Google Scholar 

  32. West NA, Kullo IJ, Morris MC, Mosley TH. Sex-specific associations of inflammation markers with cognitive decline. Exp Gerontol. 2020;138:110986.

    Article  CAS  PubMed  Google Scholar 

  33. Darweesh SKL, Wolters FJ, Ikram MA, de Wolf F, Bos D, Hofman A. Inflammatory markers and the risk of dementia and Alzheimer’s disease: a meta-analysis. Alzheimers Dement J Alzheimers Assoc. 2018;14:1450–9.

    Article  Google Scholar 

  34. Barnes LL, Shah RC, Aggarwal NT, Bennett DA, Schneider JA. The Minority Aging Research Study: ongoing efforts to obtain brain donation in African Americans without dementia. Curr Alzheimer Res. 2012;9:734–45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Bennett DA, Buchman AS, Boyle PA, Barnes LL, Wilson RS, Schneider JA. Religious orders study and rush memory and Aging Project. J Alzheimers Dis JAD. 2018;64:161–89.

    Article  Google Scholar 

  36. Wilson RS, Boyle PA, Yu L, Barnes LL, Sytsma J, Buchman AS, et al. Temporal course and pathologic basis of unawareness of memory loss in dementia. Neurology. 2015;85:984–91.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Bennett DA, Wilson RS, Schneider JA, Evans DA, Beckett LA, Aggarwal NT, et al. Natural history of mild cognitive impairment in older persons. Neurology. 2002;59:198–205.

    Article  CAS  PubMed  Google Scholar 

  38. Bennett DA, Schneider JA, Aggarwal NT, Arvanitakis Z, Shah RC, Kelly JF, et al. Decision rules guiding the clinical diagnosis of Alzheimer’s disease in two community-based cohort studies compared to standard practice in a clinic-based cohort study. Neuroepidemiology. 2006;27:169–76.

    Article  PubMed  Google Scholar 

  39. Laird NM, Ware JH. Random-effects models for longitudinal data. Biometrics. 1982;38:963–74.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We are grateful to the participants in the Religious Order Study, the Memory and Aging Project and the Minority Aging Research Study. We thank Roxann Ashworth and the team at the Genetic Resources Core Facility (RRIC SCR 018669), Johns Hopkins Department of Genetic Medicine, Baltimore, MD. The immunoassay data described here were gathered on a Mesoscale Discovery MESO QuickPlex SQ 120MM Multiplex Reader funded by National Institutes of Health grant 1S10OD032154.

Funding

Bright Focus Foundation Research Award (PMA), the Johns Hopkins University Claude D. Pepper Older Americans Independence Center, which is funded by the National Institute on Aging of the National Institutes of Health under award number P30AG021334 and NIH Grants R01AG046441 and K23 AG035005, and R01AG17917; and the Nathan W. and Margaret T. Shock Aging Research Foundation, Nathan Shock Scholar in Aging (PMA). This study was also supported by the National Institute on Aging Translational Aging Research Training Program (T32AG058527, LSN), National Institute on Aging Epidemiology and Biostatistics of Aging Program (T32AG000247, DF), K01AG050699 (ALG), P30AG10161, P30AG72975, R01AG15819, R01AG17917, U01AG46152, U01AG61356 (DAB).

Author information

Authors and Affiliations

  1. Division of Geriatric Medicine and Gerontology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Lolita S. Nidadavolu, Yuqiong Wu, Alden L. Gross, Jeremy D. Walston, Esther S. Oh & Peter M. Abadir

  2. Johns Hopkins University Center on Aging and Health, Baltimore, MD, USA

    Danielle Feger, Diefei Chen & Alden L. Gross

  3. Rush Alzheimer’s Disease Center, Rush University Medical Center, Chicago, IL, USA

    Francine Grodstein & David A. Bennett

  4. Division of Geriatric Medicine and Gerontology, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD, 21224, USA

    Esther S. Oh & Peter M. Abadir

Authors
  1. Lolita S. Nidadavolu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Danielle Feger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Diefei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuqiong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Francine Grodstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Alden L. Gross
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David A. Bennett
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jeremy D. Walston
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Esther S. Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Peter M. Abadir
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

LSN, PMA, ESO, DAB conceived and designed the study. LSN, YW performed the experiments. DF and DC performed the statistical analysis with help from ALG. LSN wrote the manuscript with input from all the authors. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Esther S. Oh or Peter M. Abadir.

Ethics declarations

Ethics approval and consent to participate

This work was approved by the IRBs of the Johns Hopkins University School of Medicine and the Rush University Medical Center.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nidadavolu, L.S., Feger, D., Chen, D. et al. Associations between circulating cell-free mitochondrial DNA, inflammatory markers, and cognitive and physical outcomes in community dwelling older adults. Immun Ageing 20, 24 (2023). https://doi.org/10.1186/s12979-023-00342-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12979-023-00342-y

Keywords

Immunity & Ageing

ISSN: 1742-4933

Contact us